Industrial development coupled with population growth had resulted in the over exploitation of natural resources. Life support systems viz, water, air and soil are thus getting exposed to an array of pollutants, especially heavy metals released by anthropogenic activities. But tolerant species of aquatic plants are able to survive and withstand the pollution stress and serves as a pollution indicators and as tools for p1hytoremediation of heavy metals from the aquatic ecosystems. Phytoremediation is an biogeotechnological application based on __ampersandsignldquo;Green liver concept__ampersandsignrdquo; and operates on biogeochemical cycling. The present study focuses on copper toxicity on morphology, biochemical parameters and bioaccumulation potential of Pistia. The laboratory experiments were conducted for the assay of morphological index parameter (MIP), biochemical parameters and accumulation profile of copper in the test plants at various concentrations viz, 2, 5,10, 15 and 20 ppm, at 4 days regular intervals for 12 days exposure. The test plants show visible symptoms like withering of roots, chlorosis, necrosis and lower leaves gets decayed at higher concentrations (severe at 20 ppm), however, the test plant showed normal growth at lower concentration viz, 2 and 5 ppm. The estimation of biochemical parameters viz, total chlorophyll, protein and carbohydrates of test plants showed significant increase at lower concentrations (2 and 5 ppm) of Cu. The biochemical constituents decreased with increase in exposure concentrations (10, 15 and 20 ppm) and duration. The toxic effect of sewage was directly proportional to its concentrations and exposure duration. The accumulation profile of Cu by Pistia was maximum at 4 days exposure and gradually decreases at subsequent exposure duration.