Aim: The aim of this current investigation is to evaluate the in-vitro antioxidant by various antioxidant assays and also the study, an attempt has been made to develop simple, precise and accurate HPTLC method Materials and Methods: to evaluate the in-vitro antioxidant by various antioxidant assays such as DPPH scavenging, nitric oxide scavenging, and also the study, an attempt has been made to develop simple, precise and accurate HPTLC method by using Rutin, Gallic acid and quercetin as a standard marker compound with mobile phase of Toluene-Ethyl acetate-Formic acid-Methanol(3:6:1.6:0.4). The detection of Rutin, Gallic acid and quercetin were performed at 254 nm respectively. Results: The ability to scavenge the free radical, DPPH was measured at an absorbance of 517 nm. So the DPPH % inhibition of ethanolic extract of A.Communis plant showed that IC50 values 831.36__ampersandsignmu;g/ml (r2=0.9957) and Nitric oxide scavenging assay showed that IC50 values938.92__ampersandsignmu;g/ml and 805.85__ampersandsignmu;g/ml (r2=0.9766) respectively as compared to the standard of Ascorbic acid of 43..71__ampersandsignmu;g/ml (r2=0.9908) The detection of Rutin, Gallic acid and quercetin were performed at 254 nm respectively. Conclusion: From the present work we conclude that species of A.Communis are highly potential in biological activity. The preliminary screening of the samples revealed the presences of high value class of compound like phenolic group as the major content in the plants. In terms of the Antioxident activity of A.Communis possess higher activity