A selective and sensitive reverse phase high-performance liquid chromatographic (RP-HPLC) method has been developed for the analysis of Clopidogrel in pharmaceutical dosage form. The elution of Clopidogrel and Diazepam (Internal Standard) was achieved using octadecyl C 18 column (4.6 i.d. / 5__ampersandsignmu; particle size/ 250 mm length) as stationary phase. The mobile phase consisted of Methanol: Water {pH adjusted to 3.5 with 0.1% Acetic Acid} (95:05, v/v) as eluents at a flow rate of 1.0 mL min-1. Detection was carried out at 220 nm. Quantitation was done by external standard calibration method. The retention time of Diazepam (IS) and Clopidogrel bisulphate was found to be 3.39 and 4.41 minutes respectively. The method has been validated for linearity, specificity, robustness, accuracy and precision. Linearity for Clopidogrel bisulphate was in the range of 10-100 __ampersandsignmu;g mL-1. The total run time of analysis was 6 minutes and the lower limits of detection (LOD) and Quantification (LLQ) were 0.020 and 0.050 __ampersandsignmu;g mL-1, respectively. This validated HPLC method using a simple mobile phase is sensitive enough for future monitoring Clopidogrel in biological samples.