Ascidians are sessile, conspicuous and important members of shallow benthic communities having rich source of nitrogenous secondary metabolites and of peptides and alkaloids. In the present study, biofoulant ascidian, Polyclinum madrasensis was collected from the pearl oyster cages of Tuticorin coast. The protein content was showed 790 __ampersandsignmu;g/ mL of crude extract and molecular weight determination using SDS page indicated well defined bands ranging from 21 to 90 kDa. The methanol and 1:1 Methanol/ Dichloromethane extracts exhibited strong hemolytic activity on chicken, goat, cow and human erythrocytes (O, A, B and AB groups). The methanolic extract the highest cytotoxicity in brine shrimp (LC50 = 97 __ampersandsignmu;g/mL). Different polar and non polar solvent extracts were assayed for antibacterial activity against ten human pathogens. The crude compound was characterized by 1H NMR and results represented.