Research on adult stem cells has been a great deal of excitement. The candidate stem cells present in the adult tissues are the hematopoietic and mesenchymal stem cells. However, there exist other heterogenous cell such as side population (SP) and endothelial progenitors (EP) that has the properties of repair and regeneration. Much work pertaining to these subpopulations is at its infancy and more research in clinical practice is of utmost important. Thus, the objective of this work is to find out whether subpopulations exist in bone marrow in higher percentage and play a vital role in tissue repair and regeneration. To test this hypothesis, we characterized stem cell populations versus subpopulations including EP cells and SP cells from Bone Marrow samples (n = 5). Surprisingly, we found that the mean of Endoglin CD105+ CD34-, CD105+ CD90 - for EP cells was found to be 41.68% and 35.88% respectively. The mean of ABCG2+ and CD117+ SP cells was found to be 3.58% and 4.34%. These results confirm the hypothesis and emphasize on the fact that migration at the site of injury in vivo and recovery not only depends on the candidate stem cells but also require the heterogenous subpopulation cells to regulate the candidate stem cells because of the wide plasticity and common precursor called hemangioblast. Thus, we conclude that the subpopulations of especially ABCG2 + and CD105+ found to be a potent source of repair mechanism and hence need to be more focused on research.