A chimeric cry2A gene of Bacillus thuringiensis (Bt) was constructed by domain swapping. DNA fragment corresponding to domain I __ampersandsignamp; II of Cry2Aa and domain III of Cry2Ac was amplified by PCR and cloned individually in a separate pBluescript vector. The cloned DNA fragments of cry2A genes were aligned to construct a chimeric cry2Ax2 gene. The chimeric cry2Ax2 gene was cloned in an E.coli-Bt shuttle vector in between cry3Aa promoter and cry2Aa terminator, for gene expression studies. Though the presence of chimeric cry2Ax2 gene has been verified by PCR, its expression in the transformants of acrystalliferous Bt strain, 4Q7, was not obvious in SDS-PAGE analysis. The non-expression of the chimeric cry2Ax2 gene may be attributed to the biasness of the new codons introduced during the process of its construction. Expression of the newly constructed chimeric cry2Ax2 gene may be achieved in the recombinant Bt strain after site directed mutagenesis or by constructing the gene by a suitable promoter in E. coli. After getting the chimeric gene expressed, it can be used for further bioassays to assess its toxicity against lepidopteron pest.