The Diclofenac sodium containing marketed gel, an emugel formulation and a spray formulation were applied on the skin. The release of Diclofenac sodium from transdermal gels and spray formulations was studied using the Tape stripping method. Diclofenac sodium exhibited distinct __ampersandsignlambda;max in methanol at 285nm. A good linear relationship (r2=0.9787) was observed between the concentration ranges of 5-25 pg/mL. The relative standard deviation (RSD) and assay values obtained by two analysts were 0.36 and 99.50, 0.31 and 99.60, for D1 formulation, 0.53 and 98.21, 0.45and 98.23 for D2 formulation and 0.54 and 97.76, 0.30 and 97.83 for D3 formulation respectively. The percutaneous absorption of D1 formulation was found to be more than other two formulations. The percentage recovery values indicates that there no interference from the excipients present in the formulation. This demonstrates that the developed spectroscopic method is simple, accurate and reproducible and can be easily used for the routine quality control of Diclofenac sodium in human stratum corneum by tape stripping method.