A three arm observational study was conducted by SRM Clinical Research Services Pvt. Ltd. to compare the efficacy of NSAIDs, serratiopeptidase enzyme and the combination of the two in relieving of pain of the root canal patient in the Aurangabad (MS) region. The study was conducted on 45 patients who were provided with a pain scale to keep a record of their rate of pain at the dosing time as well as at an hour from dosing. The rate of pain for each day was recorded in the patient__ampersandsignlsquo;s diary provided to the patients. The data was recorded from the time the root canal was opened till it was operated which is usually about 4-5 days. The data was then analyzed using statistical method and was finally represented as a histogram for easy comparison. The comparison was done for all the three arms i.e., patients prescribed with only NSAIDs, only serratiopeptidase and a combination of both the drugs. The histogram clearly indicated the change in level of pain in the patients. It was observed that the pain decreased in level when the patients consumed only NSAIDs or only serratiopeptidase enzyme but the pain was found to have aggravated in some patients of Aurangabad region when combination of both NSAIDs and serratiopeptidase was prescribed to them, hinting at an antagonistic action of the two.