Introduction: The Cystic artery is the main blood supply of cystic duct and gall bladder. If there is damage to the cystic artery during surgery may lead to serious complications, hence the origin and the course of cystic artery is important. Objective: To evaluate the variations in origin of cystic artery, relation with the Calot__ampersandsignrsquo;s triangle, common hepatic duct and cystic duct. Method: It is cross-sectional study was conducted over a period of 2 years on 40 Indian formalin fixed cadavers from Departments of Anatomy. Result: Out of 40 cadavers, cystic artery (CA) was found to be originating from the right hepatic artery in 28 cases (70%), from the left hepatic artery in 2 cases (5%), from gastro duodenal artery in 4 cases (10%), from proper hepatic artery in 3 cases (7.5%), from Superior mesenteric artery in 2 cases (5%), 1 case from right gastric artery (2.5%). It was found that in all cadavers cystic artery and cystic lymph node was found as a content of Calot__ampersandsignrsquo;s triangle. In 54 cases (90%) cystic arteries were posterior to common hepatic duct and in (10%) it was anterior to the common hepatic duct. In two cases (3.33%), cystic arteries were found anterior to the cystic duct. Superficial and deep branch of the cystic artery was found in one case. Conclusion: Anatomical knowledge of cystic artery is necessary to prevent any iatrogenic complications during surgery.