In a wide search program towards an efficient antibacterial, anti-inflammatory and analgesic agents, a series of 3,5-diaryl-1-phenyl-2-pyrazoline were synthesized starting from substituted __ampersandsignalpha;, __ampersandsignbeta; unsaturated carbonyl compounds which undergo cyclization reactions with phenylhydrazine. The synthesized compounds were characterized and confirmed on the basis of FT-IR, 1HNMR, 13CNMR and mass spectral data. The synthesized compounds 3,5-diaryl-1-phenyl-2-pyrazolines had shown significant activity against Staphylococcus aureus (MTCC 87), Escherichia Coli (MTCC 40), Pseudomonas aeruginosa (MTCC 424) and Proteus vulgaris (MTCC 426) by cup plate method using tetracycline-SD 037 as a reference standard. The anti-inflammatory property of 1,3,5-diaryl-1-phenyl-2-pyrazolines were screened by using carragenan induced paw edema method in Wistar rat. The compounds exhibited significant anti-inflammatory property further evaluated for analgesic activity using both acetic acid-induced abdominal writhing method and hot plate method respectively in Swiss albino mice. The anti-inflammatory and analgesic activities exhibited were comparable to that of the standard drug diclofenac. The safety of 3,5- diaryl-1-phenyl-2-pyrazolines were reflected by toxicity studies.