Objectives: To find out the effect of single bout of aerobic and resistance exercise on reaction time and working memory in normal subjects. Design: 30 under graduate both gender students were selected randomly using a stratified sampling method and divided into 3 groups of 10 subjects respectively. Subjects were given the working memory task extracted from the modified Sternberg test and the reaction time task. Subjects were tested prior to the exercise and took pre test values. Group 2 was given resistance exercise with multi gym and Group 3 was given aerobic exercise with tread mill walking with proper rest intervals while the Group 1 was kept as control. After the exercise was performed, they were tested again for working memory with differently prepared sheets and reaction time. The exercise given to the subjects were prolonged for 30 minutes. All subjects were given a ten minutes warm up and a cool down exercises. Result: Statistical analysis using paired t test showed mean and standard deviation for reaction time for Group 1: 0.2290__ampersandsignplusmn; 3.281, 0.2230__ampersandsignplusmn;2.983, and 0.2140__ampersandsignplusmn; 3.688 as pre, post and after 30 minutes respectively with p value ? 0.668, 0.350 between pre and post, and pre and after 30 minutes respectively. For Group 2: 0.2270__ampersandsignplusmn; 3.592, 0.2030__ampersandsignplusmn;4.547, 0.2090__ampersandsignplusmn; 4.383 as pre, post and after 30 minutes respectively with p value ? 0.158, 0.331 between pre and post, and pre and after 30 minutes respectively. For Group 3: 0.2150__ampersandsignplusmn; 3.206, 0.2250__ampersandsignplusmn;2.915, 0.2140__ampersandsignplusmn; 3.921 as pre, post and after 30 minutes respectively with p value ? 0.472, 0.924 between pre and post, and pre and after 30 minutes respectively. Conclusion: There was no statistically significant difference in the effect of single bout aerobic and resistance exercise on reaction time and working memory after single bout of aerobic and resistance exercise in normal subjects.