The present study was made to find the PvuII restriction enzyme polymorphism of lipoprotein lipase (LPL) gene and its association with angiographically defined coronary artery disease (CAD). Lipoprotein lipase (LPL) plays a central role in lipid metabolism by hydrolyzing triglyceride in chylomicrons and VLDL. Polymorphic variants of the LPL gene are common and might affect risk of CAD.Two milliliter of blood samples were collected from 30 patients, and 30 control subjects. Genomic DNA was isolated from the blood samples and subjected to PCR-RFLP analysis for determination of the genotype with regard to the Pvu II polymorphism of LPL gene. The amplified product was digested with restriction enzyme Pvu II and genotyped by through electrophoresis in 2.5% agarose gel. For the PvuII genotypes, within the CAD group(n=30), the +/- genotype (+ presence of Pvu II restriction site, - absence of Pvu II restriction site) was found in 12 individuals (40%),whereas 10(33%) carried the +/+,and 8(27%) carried the -/- genotype. In the control group (n=30), the -/- genotype was found in 6 subjects (20%), 13 (43%) carried the +/+ genotype and 11(37%) carried the +/- genotype. The genotype frequency distribution was significantly different in the CAD and control study group. The most frequent genotype among CAD patients was (-/-), this genotype was more frequent in patients than in control subjects.There was a difference in the distribution of LPL-PvuII genotypes between the healthy subjects and the patients with CAD. The common LPL polymorphic allele, the PvuII (+) or (-) is modestly associated with CAD. Genetic variants of LPL deserve further evaluation as risk factors for CAD.