Background: Urinary tract infection (UTI) is one of the most common bacterial infections encountered by clinicians in developing countries. Escherichia coli is the most common causative organism of UTI. Development of resistance by E.coli towards different antimicrobial agents is alarming. Hence, our study was planned to analyze the antimicrobial resistance pattern of E.coli isolates at a tertiary care teaching hospital. Materials and method: Culture sensitivity reports of all urine samples sent to microbiology department of a tertiary care teaching hospital during the period of July 2010-June 2013 were screened. Detailed reports were collected for all the samples in which E.coli was identified as a causative organism. Culture sensitivity testing was done by modified Kirby-Bauer disk diffusion (high media) method. Result: E.coli was isolated in total 1155 urine samples during the period of three years. Majority of patients belonged to pediatric age group (823/1155, 71.25%). 52.21% samples were of female patients. Thirty-one antimicrobial agents were tested for 13048 times for their sensitivity towards E.coli. Antimicrobial resistance ranging from 14.58% to 100% was noted among various antimicrobials. E.coli showed 38.23% resistance towards aminoglycosides, 52.27% resistance towards quinolones, 54.95% resistance towards beta-lactams and 67.33% resistance towards miscellaneous group of antimicrobials. Conclusion: Proper selection and wise use of available antibiotics will help in reducing the rate of increase in resistance. Periodic monitoring of antimicrobial susceptibility pattern of causative agent in a particular setting will be helpful in guiding judicious use of antimicrobial agents limiting the spread of resistant strains.