Background __ampersandsignamp; Objective: Maintenance of flexibility is an essential component of any conditioning program in developing joint mobility, improving athletic performance and preventing injuries. Good flexibility also provides relaxation, ease muscle pain, helping quick recovery, reducing stress, keeping the body feeling loose and agile. When soft tissue adaptively shortness overtimes muscle strength can also be altered and length tension relationship of the muscle also altered. As the muscle shorter it no longer is capable of introducing peak tension and develops tight weakness. Flexibility in hamstring muscle group is necessary for the knee extension as well as many functional activities and in prevention of injury in which the muscle group is elongated over hip and knee simultaneously. The study is aimed to compare the effectiveness of Mulligan Bent Leg Raise technique and passive stretching on hamstring tightness. Materials and Method: An experimental study was done to compare the Mulligan Bent Leg Raise (MBLR) technique and passive stretching on hamstring muscle tightness. The study was conducted in a physiotherapy college in Vadodara, India and ethical clearance was obtained from Institutional Review Board. Sixty healthy female volunteers after signing informed consent form within the age of 20 - 30 years with tight hamstring muscles were randomly assigned into two groups. Group-1, Receiving Mulligan Bent Leg Raise technique and Group-2, receiving passive stretching with knee extension. Straight Leg Raise was measured with measure tape in centimeters from tip of the heel perpendicular to the plinth before and immediately after intervention. Paired and independent t-test was used to analyze data for significant improvement and homogeneity between groups. Conclusion: This study concludes that there is significant improvement in Straight Leg Raise in both the techniques but Mulligan Bent Leg Raise Technique is significantly more effective than passive stretching in healthy females with Hamstring muscles tightness or limited Straight Leg Raise.