In recent years, degradation of freshwater ponds, an important habitat of biodiversity, caused by the anthropogenic factors, has prompted scientists from the Mediterranean countries to evaluate freshwater ponds by the IUCN Red List as threatened ecosystem [1,2]. In 2015 IUCN Red List officially gave the freshwater ponds of the Mediterranean Sea coastal area global status [1,2]. The aim of the paper is to study freshwater species: Marsilea quadrifolia and Salvinia natans. The subject of the present study was freshwater ponds of Nature 2000 and Emerald Network coastal zone with predominance of Salvinia natans and protected by the EUNIS system and Bern Convention from Sarfi to Anaklia. Methodology: During the study a transect method was used; special attention was paid to the habitat type, its ecological state, plant covering density (in %), composition of species and their quantity. Results: Freshwater ponds of the Kolkheti costal area are valuable for being an important habitat for IUCN Red List species: Salvinia natans (LC) and Marsilea quadrifolia (LC). But these habitats and, respectively, plant species are affected by the greatest anthropogenic factors such as implementation infrastructural projects (seaports, terminals) causing their degradation and disappearance. Discussion and Conclusion: In the article spreading of the habitats of Salvinia natans and Marsilea quadrifolia and threats are determined; recommendations for in-situ __ampersandsignamp;ex-situ conservation of habitats and plant species are given.