Arbuscular mycorrhizae (AM) are one of the most widespread and common type of symbiotic associations. Equisetum arvense L. is an important medicinal species of seedless vascular plants used in Khasi Hills under Nongkhyllem Reserve Forest, Nongpoh, Meghalaya, India. The mycorrhizal status of this species is meager and scarce in literature. In this study, typical AM structures were observed in sporophytes. The percentage of root length colonized by AM fungi was ranged from 80-90%. The morphological AM colonization pattern was typical Paris-type in rhizome part whereas in young roots (rhizoids), an Intermediate type of infection (both Paris and Arum types) was present. True vesicles were not seen but typical arbusculate and vesciculate structures, microsclerotia and cynophycean filamentous/trichomes structures were seen associated in the rhizoids of sporophyte. Dark septate endophyte (DSE) infection was only seen in rhizome part of sporophyte rather than rhizoids in which fine endophytes as well as endomycorrhizal infection were present. This study depicts that Equisetum rhizoids host abundant and diverse endorhizal fungal associates and also the evolution of Equisetum arvense sporophyte from hydric to mesic habitation due to its typical Intermediate type of endophytic and symbiotic association.