Objectives: Oxidative stress an unavoidable consequence of oxygen metabolism in aerobic cells is postulated to be one of the most important causes of age related changes. The changes induced by free radicals are believed to be the major source of ageing, disease development, destruction of normal cell function and membrane rigidity. The present study was intended to determine the effect of chloroform extract of Solanum trilobatum (CST) on membrane surface charge density in aged rat erythrocytes upon oxidative stress. Methods: The leaves of Solanum trilobatum were subjected to chloroform and the extract was prepared. Chloroform extract of Solanum trilobatum (CST) (150 mg/day/kg body weight) was administered orally for 90 days to young and aged rats. The erythrocyte membrane was isolated and was used to perform the studies such as estimation of protein carbonyls, glycoproteins, surface charge and enzymatic and non enzymatic levels. Key findings: A noteworthy decline in surface charge levels with simultaneous increase in protein carbonyls and shrink in glycoprotein and antioxidants status was prominent in erythrocytes of aged rats contrast to young rats. Administration of CST improved the erythrocyte surface charge density to near normalcy in aged rats. In addition a decrease in protein carbonyls level and increase in glycoproteins as well as antioxidant status was observed in aged rat erythrocytes on CST therapy. Conclusions: Therefore the polyphenols and flavanoids are the phytochemical compounds in CST that might play a central role in defending the oxidative stress related loss of membrane surface charge in a way maintaining the erythrocyte membrane integrity and functions in aged individuals.