Bacopa monnieri has been used in ayurvedic system of medicine for centuries. It is great neurotonic, immuno-modulator, adaptogen, tranquilizing, memory and learning enhancing, cerebral activator, antiulcer, anti-asthmatic ayurvedic herb. Thus the micro-propagation of this plant is very necessary to maintain the commercial demand and to preserve the germplasm of this endangered plant. The objective of this study is to determine the easy and cost effective micro-propagation protocol. Direct shoot regeneration was obtained on MS basal medium supplemented with different PGRs. Highest no. of micro-shoots (128) produced on BAP (0.1 mg/l) using leaf explant which had been previously inoculated on BAP (2.0 mg/l) i.e. shoot initiation medium. However nodal segment gave 88.2 shoots on BAP (0.5 mg/l), longer shoots (13.0cm) obtained on BAP (1.0 mg/l) + Kn (0.5 mg/l) using leaf explants. Best response in in vitro rooting (13.1) achieved on MS __ampersandsignfrac12; + IBA (1.5 mg/l) and longest roots (6.4) occurred on MS __ampersandsignfrac12; + NAA (1.0 mg/l). Rooted plantlets after acclimatization were transferred to green-house conditions they show 100% survival rates. A key step in the analysis of plant DNA restriction fragments is the purification of sufficient quantity of good quality DNA from fresh and healthy plant tissues, this technique is ideal for isolation of DNA from Bacopa plants and the isolated DNA was used for RAPD analysis. We used six primers in this analysis, all the profiles of DNA for all the smaples, were gave similar results. This indicates that the abscence of intraspecific polymorphism among the samples.