The symbiosis between AMF and wild Juniperus procera was studied in the soil of Al-Baha, Saudi Arabia. In this study, the effect of soil pH, moisture and organic matter (OM) on the colonization of AMF was investigated. J. procera infection by AMF was of the Paris type in which coarse hyphae occupied inter- and intracellular cortical cells. The spores in the soil were identified as Glomus sp, Gigaspora sp. and Acaulospora sp. Percent root colonization with AMF was positively related to pH value (r2= 0.81) and moisture value (r2= 0.75). Nevertheless, AMF colonization in the roots was lower with higher OM content (r2= 0.80). It is important to ensure that soil conditions and properties are maintained in such a way that encourages the growth of AMF and the plant. The use of AMF may be an important consideration in the replanting of this endangered plant species in the future.