The production of enzymes by bioprocesses is a good alternative to add value to agro industry residues. The aim of this work was to utilize different agro industrial byproducts as a substrate for L-asparaginase production and optimize the fermentation condition in solid state fermentation using Aspergillus oryzae. Production parameters were optimized as incubation period (96 hours), initial moisture content of 40% with inoculum size of 3%. Incubating temperature of 30 __ampersandsigndeg;C and pH 6 was found to be the best for Lasparaginase production. Among different carbon source supplemented, glucose showed enhanced enzyme production (41.33 __ampersandsignplusmn;0.80). Addition of soy bean also increased the L-asparaginase production (45.51__ampersandsignplusmn; 1.18). There were no reports on production of L-asparaginase by using pine apple peel as substrate.