Early diagnosis is crucial for disease treatment and control as it reduces the inappropriate use for antimicrobial therapy and surveillance activity. This requires the ability to detect and accurately diagnose infection at or close to the source/outbreak with minimum delay the need for specific, approachable pointof- care diagnosis able of selective between Causative Pathogens and their subtypes. Nothing of the available Pathogen diagnostic assays combines a point-of-care format with the complex capability to identify a large repertoire of human and. The ability of the microarray to identify divergent Pathogens is based on a balance between the stringency of the hybridization conditions and the signal of the virus relative to background noise on the array. Although Microbial microarrays can test for all Pathogens simultaneously, the requirement for nonspecific amplification by random priming reduces their analytical sensitivity, as compared with assays that detect a single or few agents at one time. This review discusses the applications of microarray technology has turn out to be a shows potential new tool for the detection and identification of Microbial pathogens in human Serum, plasma and cell cultures.