Tasar silk production is one of the major agro based industries playing an important role in the rural economy of the NE states of India. The hard and compact oak tasar (Antheraea proylei J.) cocoons are difficult to reel due to presence of high amounts of protein-tannin complexes. The chemical and commercial enzymatic methods developed for reeling these cocoons has drawbacks like low reeling performance, low fibre strength, high costs, low fuel efficiency and prolonged cooking time. In the present investigation, an alternative enzymatic method using pineapple (Ananas comosus (L.) Merr. cv. Queen) extract which is abundantly grown in the NE C for one hour and subsequent single silk filament reeling on an epprouvette machine and tensile testing on instron tensile strength tester. Results indicated good reeling performances with fibre strength of 2.03 0.07 g/D. Scanning electron microscopic studies showed that the silk filaments were sufficiently free from the silk gum sericin and no surface damage characterized by rupture/fibrillation of filament was seen due to excessive removal of sericin. These results showed that pineapple extract which is rich in cysteine endopeptidases can cleave the internal peptide bonds in an amino acid chain effecting partial solubilisation of the proteinaceous silk gum (sericin) involved in binding the silk (fibroin) strands together in silkworm cocoon.