Genetic variation of cultivars is very interesting in reducing genetic vulnerability as well as stabilizing production. In this regard, a study was undertaken to evaluate the genetic diversity among five accessions of Trigonella viz., IC-143851 (A1), IC-144225 (A2), IC-332236 (A3), IC-371755 (A4) and IC-433589 (A5). For the first assay, mitotic index and protein estimation were evaluated. A1 accession had the highest mitotic index (15.40%), while A4 accession had the lowest (6.83%). Highest and lowest protein contents were observed in case of A3 (31.0__ampersandsignplusmn;1.16) and A5 (23.2__ampersandsignplusmn;0.80) accessions, respectively. Total seed storage protein profiles were examined using SDS-PAGE. The proteins were resolved in 27 bands with 24 polymorphic peptides. The similarity coefficient calculated on the basis of presence and absence of bands ranged from 0.23-0.55. Following the UPGMA algorithm of similarity coefficients, the accessions could be clustered into two similarity groups. Cluster 1 consisted of two accessions (A1 and A3) while the cluster 2 grouped rest of the accessions (A2, A4 and A5). Clustering based on seed storage protein profiles provides information about the phylogenetic relationship of accessions as all the accessions have at least one or more unique seed storage protein marker that can separate them from one another.