Aims and objectives: The aim of this study was to compare the clinical efficiency of a Diode laser as an adjunct to SRP with SRP alone in the treatment of chronic periodontitis patients and also to evaluate the changes in the clinical and microbiological parameters. Materials and methods: Total number of 40 deepest sites in 10 chronic periodontitis patients was selected in this split mouth designed study. In each patient upper and lower right quadrants were assigned into one group and upper and lower left quadrants into the other. Treatment for each group was decided using a coin toss method, where Group A (Control) received only conventional SRP and Group B (Test) received conventional SRP and Laser assisted pocket debridement. The clinical parameters (Plaque index, Bleeding on probing, Probing pocket depth, Clinical attachment level) were recorded at baseline and 90th day and the microbiologic assessment for Aa, Pg and Pi were done on 7th, 21st and 90th day for both the groups. Results: Out of the 10 patients, 2 patients who were irregular for the treatment protocol were excluded. When both groups were compared there was statistically significant reduction in Plaque index, Bleeding on probing, Probing pocket depth and gain in Clinical attachment level in Group B when compared to Group A. There was statistically significant reduction in Porphyromonas gingivalis from baseline to 90th day in Group B when compared to Group A. There was no statistically significant reduction in Aggregatibacter actinomycetemcomitans and Prevotella intermedia in both the groups. Conclusion: From the results observed in this study it can be concluded that use of Diode laser as an adjunct to SRP was found to be efficacious and safe.