IJCRRI Journ Cur Res ReInternational Journal of Current Research and ReviewI Journ Cur Res Re2231-21960975-5241Radiance Research Academy2046General SciencesNON-DESTRUCTIVE GENETIC SAMPLING IN TWO CYPRINID FISH SPECIES OF SOUTHERN PART OF WESTERN GHATS-INDIA MRaja.PMalaiammal.)4148This article is copyright of Popeye Publishing, 20092009This is an open-access article distributed under the terms of the Creative Commons Attribution (CC BY 4.0) Licence. You may share and adapt the material, but must give appropriate credit to the source, provide a link to the licence, and indicate if changes were made.

Non- destructive genetic sampling for DNA isolation in two cyprinid endemic fishes of the species Danio aequipinnatus and Puntius tambraparniei was attempted. The total genomic DNA was isolated from fin clips, scales, liver and muscles by using Phenol: choloroform method by different storage method like Ethanol/EDTA and air dry in the different cell lysis buffer like TNES- urea and TNES. In Puntius tambraparniei showed higher quantity of genomic DNA (402.36__ampersandsignmu;g) in the liver tissues by using the TNES cell lysis buffer in the ethanol/ EDTA storage method. Danio aequipinnatus also showed the high quantity of genomic DNA (415.26__ampersandsignmu;g) in the muscle tissue by using TNES cell lysis buffer in the Ethanol/ EDTA storage method.

Non-destructive genetic sampling DNA extraction fin clips scale muscle cyprinid fish.