Background: The search for a simple and accurate analytical method to determine the concentration of blood serum components is of major importance in clinical laboratories. Fourier Transform InfraRed (FTIR) spectrometry is a global, sensitive, and highly reproducible physicochemical analytical technique that identifies structural moieties of biomolecules on the basis of their absorption in the infra red region of the electromagnetic spectrum. Objective: Since a biomolecule is determined by its unique structure, each biomolecule exhibits a unique FTIR spectrum, representing the vibrations of its structural bonds. FTIR analytical applications have allowed determination of blood contents using a single spectral measurement. Materials __ampersandsign Methods: In this work, simultaneous determination of concentrations for the major components in human blood serum, namely glucose, protein, triglycerides, cholesterol, urea, and creatinine has been investigated using Fourier Transform InfraRed (FTIR) spectroscopy. The FTIR spectra of 35 blood samples have been recorded in the mid frequency region, 4000 – 450 cm-1. The spectral measurements of 25 samples with known clinical parameters have been employed with Linear Regression Statistical Analysis using SPSS software to quantitatively correlate IR spectral features with the clinical analytical results. Results: The resulting quantification methods have been then validated with the remaining 10 specimens. Conclusion: The scatter plots obtained has validated that IR spectroscopy has the potential to become the potential tool in the clinical laboratories for instantaneous and simultaneous determination of human blood serum testing.