Azithromycin is macrolide antibiotic is effective against a wide variety of bacteria such as Hemophilus influenzae, Streptococcus pneumoniae, Mycoplasma pneumoniae, Staphylococcus aureus, mycobacterium avium, and many others. The aim of present work was to formulate sustained released azithromycin microsphere to overcome the problem related to drug like insoluble in water, bitter taste and gastrointestinal side-effects. Microspheres were prepared by varying drug polymer ratio using emulsion solvent evaporation technique. Characterization of microspheres was done by Differential Scanning Calorimetry (DSC), X-Ray diffractometry (XRD), Surface Morphology (SEM), Stability studies.