Aim: To study the formation of median nerve by multiple lateral roots. Materials and Methods: 50 upper limbs of 25 donated embalmed cadavers (20 males and 5 females) were studied in the department of Anatomy at K. J. Somaiya Medical College, Sion, Mumbai, INDIA, the formation of median nerve by multiple lateral roots were observed. The finding was noted after thorough and meticulous dissection of the upper limbs of both sides (axilla, arm, forearm and palm) of 25 cadavers. The photographs of the variations were taken for proper documentation. Observations: The variations were found in two cases. In the first case the median nerve was formed by multiple roots- three roots from lateral cord and one root from medial cord of brachial plexus. In the second case out of the three roots forming the anomalous median nerve, two were from lateral cord and one from medial cord of brachial plexus. However, the distribution of the anomalous median nerve was normal in arm, forearm and palm. The arterial pattern in the arm (axillary and brachial arteries) was also normal in both the specimens. Conclusion: The presence of such type of variations are clinically important for surgeons, orthopaedicians and anaesthetist performing pain management therapies on the upper limb.