Aims: To study the microbial degradation of hydrocarbon from oil contaminated soil sample using Pseudomonas sp. and were analyzed the different physicochemical characters of normal and oil contaminated soil. Method and Results: The soil sample was collected from oil contaminated site, in Sivakasi and then the oil degrading microorganism (Pseudomonas sp) was screened from oil contaminated soil by using enrichment technique. After, the isolated microorganisms were identified by using standard characterization method. From the oil contaminated soil, 10 bacterial strains were isolated. Among them, tolerance of two strains was used for further studies. The physico-chemical characters of normal and oil contaminated soil samples were analyzed by standard method. In this study, the pH, moisture, carbon, phosphorous and potassium content of normal and oil contaminated soil samples were analyzed and compared. In the present investigation, the various concentration of aromatic hydrocarbon (toluene) was degraded by Pseudomonas sp and rate of the degradation was analyzed by HPLC (High Performanace Liquid Chromatography). The chemo taxis properties of the Pseudomonas aeruginosa and Pseudomonas fluorescens were studied by chemo taxis assay and also studied biosurfactant production of Pseudomonas sp. simultaneously the biomass, emulsification activities of Pseudomonas sp on toluene was studied. This biosurfactant production was detected by Thin Layer Chromatography. Conclusions: In this present investigation, the aromatic hydrocarbon degradation efficiency, chemotaxis properties and biosurfactant production of Pseudomonas sp. was compared with MTCC cultures of Pseudomonas stutzeri. Significance and impact of the study: Treatment with adapted bacteria could degrade the hydrocarbons.