The aim of the present study was to isolate Salmonella typhi from various water sources and identification of drug resistant genes cat P and tem using Multiplex PCR technique. Enteric fever is prevalent world over and continues to be a major public health problem in developing countries. Infection with Salmonella typhi, the causative organism of this disease, requires effective antimicrobial chemotherapy in order to reduce mortality. Antibiotic-resistant strains of Salmonella are now encountered frequently and the rates of multidrug-resistance have increased considerably in recent years. In the present study, 30 samples were collected from various water sources, of which Salmonella typhi was isolated from 12 samples. All the isolates were subjected to antimicrobial susceptibility testing by standard disc diffusion method against 10 antibiotics. Isolates resistant to chloramphenicol and ampicillin were selected and the drug resistant genes were identified as cat P and tem respectively by Multiplex PCR technique. There was a very good correlation between the genotypic analysis by PCR and the phenotype determined by standard methods of susceptibility testing. __ampersandsignnbsp;