Physical and chemical characteristics of oil extracted from Melia azedarach L. seeds are presented in this work. The results indicate that Melia azedarach seeds constituted 39.6% crude oil. The specific gravity, refractive index, saponification value, and unsaponifiable matter content of Melia azedarachseed oil were found to be 0.80 g/cm3, 1.36 40 D n , 84.15 (mg KOH/g) and 0.71 (%w/w) respectively. Elemental analysis of oil by AAS and Titration method shows that it contains Ca (1230 mg/100g), Mg (990 mg/100g), K (121 mg/100 g), Zn (3.12 mg/100 g), Mn (3.4 mg/100g), Fe (19.52 mg/100g) and P (213 mg/100g). The extracted oil was methyl-esterified and analyzed by GC and GC-MS. The major fatty acids reported here, in Melia azedarach seed oil, are Palmitic acid (5.68%), Linoleic acid (74.57%), Oleic acid (16.39%), Stearic acid (3.33%). This work might be useful for developing applications for Melia azedarach L. seed oil.