Aim: The aim of this study is to evaluate the association between urinary crystal and urinary tract infection (UTI) in patients attending Saveetha Medical College and Hospital. Method: A cross-sectional study was conducted with 1500 urine specimens. A total of 1500 urine specimens received to microbiology and pathology laboratory were included in the study. Urine with crystals were analysed for culture positivity. Urine routine examination was done for urine crystals analysis. Semi-quantitative method (standard loop technique) was done for analyzing significant bacteriuria. The correlation between the urinary crystal and urinary tract infection were analysed by odds ratio. Results and Conclusion: A cross-sectional study was conducted with 1500 urine specimens. A total of 1500 urine specimens received to microbiology and pathology laboratory were included in the study. Urine with crystals were analysed for culture positivity. Urine routine examination was done for urine crystal analysis. Semi-quantitative method (standard loop technique) was done for analyzing significant bacteriuria. The correlation between the urinary crystal and urinary tract infection were analysed by odds ratio. Out of 1500 urine sample, 155 were found to be crystal positive, in which bacterial isolation rate was more from uric acid crystal positive samples. Escherichia coli was the maximum yield from the crystal positive samples with the colony forming unit (cfu) of 10000 cfu per mL (less than significant bacteriuria of 1,00,000 cfu/mL). Other organisms isolated were Klebsiella spp, Pseudomonas spp, Citrobacter spp, Enterobacter spp, Acinetobacter spp. and Proteus spp. Their resistance pattern was also analysed.