Serum adenosine deaminase enzyme and Serum ferroxidase and albumin levels were determined in 50 newly diagnosed patients of tuberculosis, as Case. Fifty age and sex matched healthy individuals were taken as controls. Mean __ampersandsignplusmn; SD of serum adenosine deaminase and serum ferroxidase in controls and case was found to be 22.90__ampersandsignplusmn;3.87U/L,873.82 __ampersandsignplusmn; 117.44 IU/L, and 56.73__ampersandsignplusmn;14.43 U/L, 1708.74__ampersandsignplusmn;283.57 IU/L, respectively. Serum adenosine deaminase enzyme and Serum ferroxidase in case was significantly higher as compared to controls (p__ampersandsignlt;0.001). The decreased levels of serum albumin in case, as compared to control was statistically significant (p__ampersandsignlt;0.001).Serum ferroxidase: albumin ratio (Ferroxidase in International Unit per gram of albumin) in case (60.05__ampersandsignplusmn;14.46 IU /g) was significantly higher than controls (25.02__ampersandsignplusmn;4.5 IU/g), (p __ampersandsignlt; 0.001) Serum adenosine deaminase enzyme good marker for diagnosis of TB but when it combine with Serum ferroxidase: albumin ratio (IU/g) it can therefore be employed as a surrogate marker to lend a hand in diagnosis and prognosis of pulmonary tuberculosis.