The present study was conducted to determine the prevalence of urinary tract infection among diabetic patients and causative pathogens also. A total of 200, out of which 95 patients were male and 105 female patients were included in the present study. Diabetic state of the patients was assessed as per the guidelines of World Health Organization (WHO). For bacteriological study, urine samples were processed according to standard microbiological techniques. Identification of the species of microorganisms was performed by Gram__ampersandsignlsquo;s staining as well as by biochemical tests as per standard method. Only urine culture showed __ampersandsigngt;105 colony forming units (CFUs/ml), were considered for UTIs infection and processed for antibiotic sensitivity test. Here, the results indicated that the urine sample of 36.1 % female patients and 12.6 % male patients were showed significant growth in culture media. From Gram__ampersandsignlsquo;s staining and biochemical tests it may be stated that the Streptococcus faecalis, Escherichia coli, Staphylococcus epidermidis, Staphylococcus saprophyticus, Klebsiella oxitoka, Streptococcus pyogenes, Klebsiella pneumoniae, and Pseudomonas aeruginosa are present in the culture those are causative pathogens for urinary tract infection. The results of the present study enlighted that in our study area the prevalence of UTIs was high in diabetic female than diabetic male patients.