Objectives: Work, when performed in prolonged sitting position can have an impact on knee jointposition se nse (JPS) due to altered weight transmission and may contribute to early degeneration of knee joint. However no literature is available that compares the knee JPS between the women involved in prolonged sitting type of job to non-working women. Hence this study compares theknee JPS between working and non-working women to find the impact of prolonged sitting. Materials and Methods: Knee JPS was measured in both knee joints by active repositioning method with a standardized goniometer in terms ofabsolute and relative error on 80 subjects (40 in each group). Subjects were selected through random convenient sampling. Result: There were no significant differences in-terms of both absolute and relative error between the groups (P__ampersandsignlt; 0.05) in both the knees. Conclusion: This is the first study (to the best of our knowledge)`which has analysed the position sense error between the working and nonworking women group. It was found that prolonged sitting has no influence on knee JPS and this could be a least contributing factor for the development of the knee joint degeneration in any women at 40-50 years of age. side at 45__ampersandsigndeg;, 60__ampersandsigndeg; respectively. And RE of right side at 60__ampersandsigndeg; strongly correlated with RE of right side 80__ampersandsigndeg;, left 60__ampersandsigndeg;, 80__ampersandsigndeg;. Other strong positive correlations between errors of different angles are given in table- 5. DISCUSSION Joint position sense may be impaired in case of decreased feedback from the joint receptors in case of prolonged non-weight bearing positions when adopted e.g. seated office work.11,12,14 The impaired position sense could be a contributing factor for the initiation of early stage of structural damage in the knee rather being secondary to the disease.7 Also because the progressive development of articular surfaces degeneration of knee joint is solely not the disease of aging, but potentially have etiological link with the occupation.12 The aim of the study was to determine the differences in knee joint position sense among working and non-working middle aged women to see the scientific basis of posture related changes and to detect the development of early signs of degenerative changes in the joint. Hence the age range of 40 - 50 yrs was considered for the study, since the degenerative changes in the joints among women sets in at 51 yrs of age on an average;12 and the average age at which osteoarthritis becomes clinically symptomatic, was found to be 59-60 years for females involved in desk job.12 In this study the age and BMI of the subjects of both the groups were not statistically significant, (p= 1.31, 0.68) suggesting that the confounding factors were controlled. Our understanding while designing the study was that there will be subtle differences in knee joint position sense between working and nonworking females, since working women would have probably uniform sitting pattern for a fixed number of hours compared to the non-working subjects. But the results indicated that in this age range, among both the groups, changes in the joint position sense at all angles were relatively equal; Average AE of working women was 6.624__ampersandsigndeg;__ampersandsignplusmn;5.033__ampersandsigndeg; and non-working was 5.665__ampersandsigndeg;__ampersandsignplusmn;4.957__ampersandsigndeg;. Average RE of working women was 2.84__ampersandsigndeg;__ampersandsignplusmn;8.13__ampersandsigndeg; and non-working women was 2.46__ampersandsigndeg;__ampersandsignplusmn;7.185__ampersandsigndeg; (p__ampersandsignlt;0.05). Such differences in joint position sense are statistically insignificant. These changes are also clinically insignificant (minimal clinical difference was found to be 5__ampersandsigndeg; in a previous study12) suggesting that, both groups activity levels did not have any influence on knee joint position sense in this age range. However 40 - 50 years can be considered as a healthy age range, therefore we can assume that the individual may not demonstrate any initiation of changes in terms of position sense error, which can begin the process of degenerative changes physiologically.12 In this study we observed that the speed at which the subjects adopted to reposition their joints was not uniform among the subjects. Literature indicates that slower velocity can influence the position sense capacity negatively even in healthy individuals.15 But the velocity at which the joints were repositioned was not analyzed. Therefore we cannot attribute that the velocity would have any influence among the groups in order to have their joint position sense relatively uniform. Furthermore, both the AE and RE was found to be relatively uniform (larger) in the right knee at 45__ampersandsigndeg;, and left knee at 60__ampersandsigndeg; in both the groups. Such uniformity was more in the right compared to left, this we attribute on limb dominance factor. But when analysis was done for specific angle, we noticed that at 80__ampersandsigndeg;, the errors were less in both the knees. This result is in favor of the previous studies, where authors had concluded that articular afferents discharge maximally at the extremes of joint movements, and are also active within the joint?s midranges.16