Introduction: To date, there are very few reports comparing on duration of hold of end position in MFR on joint range of motion (ROM) and hamstring muscle tightness. The purpose of this study was to examine the length of time the hamstring muscles should be placed in end position of MFR. Aim of Study: To study the effect of myofascial release (MFR) in reducing the hamstrings tightness. Methodology: Study design: An experimental comparative study Sample selection: A random sample of 31 students were taken from SBB Physiotherapy college, Ahmedabad after giving due consideration to inclusion and exclusion criteria. All of them took part in study on a voluntary basis after signing consent. Sample size: Total 31.In Group A: 16 students (End position of MFR was maintained for 30 seconds) Group B: 15 students (End position of MFR maintained for 60 seconds) Inclusion criteria Normal healthy individuals, female students, age group: 16-24 years, hamstrings are tight and passive straight leg raising (SLR) range was between 30-70 ranges. Exclusion criteria Any fracture of spine or lower limb within last 6 months, tight rectus femoris and tight ilio psoas, hypersensitive to skin Outcome measure Passive Straight leg raising (SLR) test. MFR was given for total of 12 sessions with subject in prone position for 5 repetitions/session 6 days in a week for 2 weeks to both groups. Results: In Group A and Group B results showed highly significant improvement in passive SLR after 10 days of treatment at 5% level of significance. On comparing Group A and Group B the results showed no significant difference in passive SLR between both groups at 5% level of significance. Conclusion: From the present study it can be concluded that, by MFR there was significant improvement in passive SLR range. But no significant difference in improvement of passive SLR if we hold the end position for 30 seconds or 60 seconds.