Introduction: Adolescent period is a critical link between childhood and adulthood, characterized by significant physical, psychological, and social transitions. Adolescent girls are particularly vulnerable to nutritional problems and anemia is one among them. Adequate knowledge can promote good practice and help in the prevention of iron deficiency anaemia. Objectives: The study was carried out to determine the knowledge regarding prevention of iron deficiency anemia among adolescent girls and to find the association between knowledge scores and selected demographic variables. Materials and method: A descriptive survey approach was used in the study, non probability purposive sampling technique was used to select 100 adolescent girls and data was gathered using the structured knowledge questionnaire on iron deficiency anemia. Data was analyzed by using descriptive and inferential statistics. Results: The study result showed that majority (84%) of study sample had moderately adequate knowledge, 11% had inadequate knowledge and 5% had adequate knowledge on prevention of iron deficiency anaemia. There was no significant association found between knowledge scores and the selected demographic variables of the adolescent girls (p__ampersandsigngt;0.05) Conclusion: This study concluded that majority (84%) of the study sample had moderately adequate knowledge on prevention of iron deficiency anemia so it is advisable to provide educational programs for the adolescent girls regarding iron deficiency anemia.