Objective: The present study was undertaken to detect the prevalence of multiple antibiotic resistant bacteria isolated from ICU patients and to study their antibiotic susceptibility profile. Method: The organisms were isolated from different clinical specimens and antibiotic susceptibility tests were done as per Clinical Laboratory Standard Institute (CLSI) guideline. The different tests for detection of Methicillin Resistant Staohylococcus aureus (MRSA) and Gram negative bacilli producing Extended spectrum beta-lactamases(ESBL), AmpC __ampersandsignbeta;-lactamases and Metallobetalactamases(MBL) were performed by standard methods. Results: A total number of 240 bacterial strains were studied. Out of which 105(43.8 %) were Gram positive isolates and 135(56.2%) were Gram negative isolates. 57(54.3%) MRSA strains were isolated. Amongst Gram negative isolates 29(21.5%) strains were only ESBL; 13(9.6%) only AmpC __ampersandsignbeta;-lactamase, 18(13.3%) both ESBL and AmpC __ampersandsignbeta;-lactamase and 21(15.6%) were MBL producers. 19 MBL producing strains were resistant to all antibiotics studied as per CLSI guideline. Conclusion: Accurate detection of multidrug resistant bacteria by any Clinical Microbiology Laboratory should be done for prompt and effective antimicrobial therapy to ICU patients and successful management of infections.