Aim and Objectives: Leptin, a multifunctional adipokine and pleiotrophic hormone produced by the adipose tissue plays a significant role in pathogenesis of periodontitis. The purpose of this study is to compare the gingival crevicular fluid (GCF) leptin levels in obese and non-obese individuals with and without Chronic Periodontitis and to lend greater clarity to the role of leptin as a reliable biomarker for Periodontitis. Materials and Methods: Sixty subjects were divided into 4 groups of 15 each based on the clinical evaluation and body mass index (BMI) __ampersandsignndash; Group I (obese with healthy periodontium), Group II (non-obese with healthy periodontium), Group III (obese with chronic periodontitis) and Group IV(nonobese with chronic periodontitis). GCF samples (by microcapillary pipettes) were collected to estimate the levels of leptin using enzyme linked immunosorbent assay (ELISA) kit Results: The mean GCF leptin levels in Group I is 715 __ampersandsignplusmn; 71pg/ml, Group II is 503 __ampersandsignplusmn; 230 pg/ml, Group III is 346 __ampersandsignplusmn; 82 pg/ml and Group IV is 137 __ampersandsignplusmn; 40 pg/ml. It proved that Group I and II had significantly higher leptin concentrations than Groups III and IV (p__ampersandsignlt;0.05). In healthy and periodontitis subjects BMI and leptin were positively correlated. Conclusion: The results suggested that leptin being an adipokine is increased in obese subjects and GCF leptin levels are increased in healthy states. Since leptin concentration in GCF is inversely proportional to clinical attachment loss, leptin may be a prognostic marker in periodontal disease progression and in assessing post treatment outcome