Eukaryotic Aminoacyl-tRNA synthetases exist in large complex consists of different tRNA synthetases with auxiliary proteins. P43 is one of the three non-synthetases proteins found in multi-synthetases complex. P43 has been shown to involve in various biological processes like tRNA transport from nucleus, apoptosis etc. Homologous sequence of P43 is also found in Plasmodium falciparum (PfP43). In this study, homology modeling, structure validation and active site determination methods were used to perform structural characterization of P43. Results show the overall three-dimensional structure of P43 with proper Ramachandran plot. Also, Active site residues were nicely located onto the structure of P43. In addition, structural comparison between P43 of human and parasite origin provided information on subtle differences in overall structures of proteins. Our results suggest that elucidation of PfP43 structure is critical in developing anti-malarial drugs.__ampersandsignnbsp;