This research work brings to the attention of those more concern in fish issues caused by the chemical parameters on natural resource water. To carryout research on behavior / survival rate of Puti fish in Nallah water having less content of Dissolved Oxygen and low pH due to contamination of Acid mine drainage was considered. Various concentrations of synthetic acidic leachate have been used to perform the experiment. Synthetic water is prepared in laboratory for experiment. Ranvendli nallah water is collected for preparation of synthetic water and Puti fishes are collected from same nallah. pH aned Dissolved Oxygen are essential water parameters for survival of fishes in any natural resource. Experiment conducted to study fish behavior related to the concentration of the assessed parameters, pH aned Dissolved Oxygen in Six jars. The study leads to the conclusion that though acidic leachates drop the pH aned Dissolved Oxygen of natural water but fish can survive to the great extent in the same water.__ampersandsignnbsp;