Fifteen different bacterial strains were isolated from the vegetable wastes, were screened for the production of polygalacturonase enzyme. The most responding strains which exhibited maximum Polygalacturonase activity is named as PG-2 isolate. This isolate is gram negative, rod shaped and nonsporulating in nature. The cultivation of selected strain (PG-2 isolate) in liquid medium resulted in high quality of polygalacturonase enzyme. The maximum Polygalacturonase activity was reached in 72h of growth in medium having citrus pectin as carbon source and mixture of casin hydrolysate + yeast extract (1% v/v) as nitrogen source. This organism exhibited maximal Polygalacturonase activity at pH 6.5 at incubation temperature of 30oC. The PG-2 isolate exhibited maximum Polygalacturonase activity in sodium citrate buffer pH 5, when incubated at 40oC for 15 min.__ampersandsignnbsp;