The aim of the study was to evaluate hepatotoxicity and genotoxicity in patients suffering from pulmonary tuberculosis. It was carried out on the pulmonary tuberculosis male patients from directly observed therapy, short-course (DOTS) Centre Fatehpur district (U.P) India. Patients were divided into three groups- 1) Before treatment 2) After treatment and 3) Control. Toxicity levels in the blood samples were determined by standard liver function tests (ALT __ampersandsignamp; AST) by spectrophotometry. The study indicates significant increase in the level of liver transeaminases (ALT __ampersandsignamp; AST, P __ampersandsignlt; 0.05) in the after treatment group. Antioxidant analysis shows that the patients who suffer from oxidative stress have reduced Glutathione (GSH) and high Melonaldehyde (MDA) levels. After treatment with anti-TB drugs the level of GSH increases (P __ampersandsignlt; 0.05) while level of MDA decreases (P __ampersandsignlt; 0.05). Further genotoxic effect of anti-TB drugs was evaluated on lymphocytes by COMET assay which showed observable DNA damage in after treatment study group patients (P __ampersandsignlt; 0.05). LDH expression profile of different isoforms of lactate dehydrogenase(LDH) on native polyacrylamide gel electrophoresis(PAGE) indicates that in pulmonary tuberculosis patients the LDH 3 isoforms was expressed more as compares to the other isoforms which may be due to damage in the lung cell lining by the mycobacterium. In some after treatment patients LDH 5 isoform level increased, which is an indication of liver injury due to hepatotoxic and genotoxic effect of anti-TB drugs on the patients.