Objectives : This study was done to find out the seroprevalence of herpes simplex virus type 2 (HSV-2) and its association with certain factors like age , gender as well as other demographic and behavioural factors in 166 human immunodeficiency virus ( HIV ) positive adults . Methods : This is a cross sectional study . 166 HIV positive adults were selected , informed consent was obtained and detailed history was taken. Serum samples were then screened for HSV-2 type specific IgG antibodies by ELISA . Results : HSV-2 seroprevalence was 42.1 % and it was higher among women ( 56.3% ) than among men ( 31.6 % ) . HSV-2 seroprevalence increased along with advancement of age and it was associated with high promiscuous behaviour . Conclusions : Only 14.2 % of the HSV-2 seropositive group gave history of symptoms suggestive of genital herpes . This demonstrates the need for regular HSV-2 seroprevalence surveys particularly in high risk groups. HSV-2 infected individuals can therefore be identified and institution of proper counselling and treatment not only reduces the rate of transmission of HSV-2 infection but also decreases the incidence of HIV infection.