Staphylococcus saprophyticus may be present on normal human skin and the periurethral area and can cause urinary tract infection, particularly in sexually active young women. In the present study, an attempt has been made to isolate, identify and to know the antibiotic sensitivity pattern of Staphylococcus saprophyticus from urine samples collected from the same group. In the present study, a total number of 100 urine samples were collected from sexually active young women 15-40yrs of age attending the Gynaecology outpatients department, MIMS General hospital. Out of 100 samples, 88 were culture positive and 12 samples showed no growth. Out of 88 culture positive cases, 18 (20.45%) samples were positive for Staphylococcus saprophyticus. All the 18 Staphylococcus saprophyticus strains were identified by their colony morphology, biochemical tests and were subjected to antibiotic sensitivity testing by modified Kirby-bauer disc diffusion method. All the Staphylococcus saprophyticus strains isolated(n=18) were 100% sensitive to Vancomycin, 55.56% to Oxacillin and Ciprofloxacin, 50% to Gentamicin and 38.89% to Tetracycline. The resistance pattern showed by the above strains isolated was 100% to Penicillin, 61.11% to Tetracycline, 50% to Gentamicin, 44.44% to Oxacillin and Ciprofloxacin respectively. In the present study, Staphylococcus saprophyticus is the second most common organism isolated from sexually active young women suffering from symptomatic urinary tract infection which coincided with several other authors who reported the same in their studies. The present study also reveals the need to report Staphylococcus saprophyticus as a pathogen when isolated from sexually active young female outpatients suffering from symptomatic urinary tract infection.