Aim: To compare existing technique of bitemark analysis, the manual docking technique with a new novel technique using 3D scans of the bitemark region and subjecting them to overlay comparison using algorithms. Using the 3D scans, study of time related distortions in the bitemark region, with algorithm based overlay. Methodology: 30 bitemark were obtained on an inanimate object (apple) and on putty material. The dental casts of the same subjects were recorded and used for the comparison analysis. The bitemark on either material were manually docked with the dental casts of the respective subject. The 3D scans of the bitemark and dental casts were obtained for analysis using algorithm based overlay. Time related distortion (over 7 days) was analysed in the bitemark region using the algorithm based overlay of the scans obtained on day 1 and day 7. Results: The manual docking analysis results depict excellent match of the dental cats of the subject with the bitemark region, however, manual interference was seen to be high and hence not relevant. The 3D docking analysis on an inanimate object( apple) showed 80% samples to be__ampersandsignrdquo; most likely__ampersandsignrdquo; match with the respective dental cast . The 3D docking analysis of the bitemark on putty showed 63.3% match to be __ampersandsignldquo;high certainty__ampersandsignrdquo; match with the dental casts. Comparison of the distortion seen in both the material was seen to be high in the bitemark on apple; putty depicting negligible distortion. Conclusion: The newer technique of optical 3D scan analysis using algorithm based overlays shows promise and helps minimize manual interferences. Material such as Putty shows less distortion over time and can be an excellent choice for recording and storage of the bitemark samples.