Peptococcus activus are anaerobic, non motile, non spore forming gram positive cocci. The objective of this study was to investigate the availability of this bacteria and their ability to protease production. Eleven different bacterial colonies were isolated from shrimp and dry fish(shrimp).Of them, eight colonies(Z1-Z8) were isolated from shrimp and rest of the three isolated from dry fish (Z9-Z11).Among them peptococcus activus which was named Z10 before identification exhibited highest protease production at temperature 37__ampersandsignordm;C,medium pH 5.0 and 48 hours of incubation period. Maximum protease activity was achieved at 400C for 70 minutes and pH 5.0 along with 2% gelatin as substrate concentration during enzyme substrate reaction.