Various types of fungal species have been reported to be employed for production of pectinases. As extracellular pectinases are easier to harvest and thus the scale up is cheaper and simpler. Thus in the present study, kinnow waste was used to generate extracellular fungal pectinases from natural sources along withextraction of pectin. Morphological examination of screened isolates revealed that Isolate#1 and Isolate#3 could be of Rhizopusgenus and Isolate#2 could of Aspergillus genus. Aspergillusoryzaevaroryzaewas used as standard fungi in the present. Quantitative estimation of total protein was made to know total enzymatic activities generated from isolates. It was done via SmF as well as SSF and pure pectin resulted in maximum activity among substrates, followed by peel powder and finally pomace. In SmF, among all isolates, Isolate #3 was found to produce maximum total protein and all other enzymatic activities(Total protein-8.69mg/ml/10min, Pectinase-763.82 nmoles/ml/60min, Polygalacturonase activity-99.83 nmoles/ml/10min andPL-1038.68nmoles/ml/15min). Extraction of pectin resulted in 20% yield from kinnow peel powder, 80% Ethanol was found to be suitable for extraction of pectin and 7.3% calcium pectate was obtained.