Diabetes Mellitus (DM) is the most common disorder characterized by metabolic abnormalities and long term complications. Hyperglycemia is considered a primary cause of diabetic vascular complications and is associated with oxidative stress. The purpose of this study was to investigate the role of hyperglycemia in generating oxidative stress if any, in DM Type 2. The study was carried out in 55 patients of DM Type 2 and also in 50 healthy controls. The patients were divided into two groups Group I (n=35) comprising patients of DM Type 2 with out complications and Group II (n=21) comprising patients of DM Type 2 with complications. Various parameters like Adenosine Deaminase (ADA) activity which is found to be a marker of abnormal lymphocyte response and a producer of reactive oxygen species (ROS), Malondialdehyde (MDA) which is a parameter to study increased oxidative stress leading to lipid peroxidation and Superoxide dismutase (SOD) activity which is an indicator of antioxidant status were taken into consideration to establish the existence of oxidative stress in DM Type 2. Glycosylated hemoglobin (HbA1c) was also assessed to determine the status of diabetic control. The parameters like HbA1c, serum ADA and serum MDA were significantly raised in DM Type 2 cases (both Group I and Group II) when compared to controls. Likewise the mean serum SOD was significantly lower in DM Type 2 patients (both Group I and Group II) as compared to Control group. When the levels of HbA1c, serum ADA and serum MDA in Group II patients of DM Type 2 when compared with those of Group I patients of DM Type 2, they were significantly increased. Likewise the serum SOD in Group II patients of DM Type 2 registered a significant decline as compared to Group I patients of DM Type 2. These alterations in DM Type 2 especially in Group II are ascribed to increased glycation, T-lymphocyte response, oxidative stress and limited defense against the radicals ,which act as harbinger of various metabolic complications.