In accordance with the rules of Bio Medical Waste (Management and Handling), every hospital generating BMW needs to set up requisite BMW treatment facilities. Inadequate management of biomedical waste can be associated with risks to healthcare workers, patients and communities at large..Hospital infection control committee __ampersandsignamp; team are responsible for monitoring of proper implementation of biomedical waste management rules. The issue is not only related to quality waste management but also to safety of health care workers i.e. universal precautions and immunization. Objectives: To evaluate Biomedical Waste Management practice at tertiary care hospital and to find out the change, if any, in practice of biomedical waste management after training. Method: Cross sectional interventional study conducted at 48 randomly selected Bio Medical Waste Management (BMW) management stations tertiary care hospital, Ahmedabad during March 2009 to November 2009. Results: There was considerable gain in availability of personal protective devices (20.84%), reduction of over flown bags (18.75%) and availability of bags (8.33%) after training. Regarding manpower there was increased in staff vaccination (68.75% -77.08%) and awareness about post exposure prophylaxis (12.50%). Not only that there was improvement in use of personal protective devices by servants (39.59%) and reduction of sharp /needle prick injury (8.33%) after training. Conclusion: Conduction of training workshops on BMW management for Hospital staff by Infection Control Committee has shown promising results in practicing BMW management. This study recommends periodic training workshop on BMW to reinforce and update knowledge of hospital staff for proper implementation of BMW Act.